Subcloning

With our specific technology, there is only one step to subclone the target gene fragment into any site of the desired vector. At the same time, it does not rely on any restriction enzyme cut site. So it will take less time than the traditional restrictive enzyme method. This will assures us finish a lot of subclones which needed to insert different gene fragments into the same site of vector in a short time.

Request:

1. Tell us your target gene sequence through the E-mail, or tell us the gene’s ID number in NCBI databank.
2. Provide us the plasmid or DNA fragment (tell us the concentration and amount > 1ug) which contains the objective gene.
3. Provide us the desired vector (tell us the concentration and amount > 1ug) and tell us the subcloning site.
4. If you only have the target gene sequence, we can help you synthesize gene, then subclone the gene to your desired vector. If you want to know more details, you can email to us.
5. If need the endotoxin free plasmid after we finished the subcloning, please tell us the concentration and amount of recombinant plasmid.
6. term：

7. Now we accept the order (>20 clones). Please inquire about prices if you have one or few clones.

Procedure：

2. The recombinant plasmids were sequenced, subsequently the correct ones were prepared.

3. We will provide plasmids (>5ug) and glycerol strains containing the recombinant plasmids. At the same time the sequence reports and electrophoresis maps of plasmids will be sent.

4. Qualities: certified by DNA sequencing, the sequencing results match 100% to your gene.